Mismatch repair activity in mammalian mitochondria
نویسندگان
چکیده
منابع مشابه
Strand-specific mismatch repair in mammalian cells.
Mistakes occurring during DNA transactions necessarily produce mismatched base pairs. DNA biosynthetic errors that escape the polymerase editing function yield mispaired bases, but mismatches also populate a recombination heteroduplex produced by strand transfer between related sequences that have diverged at the nucleotide level. Study of microbial systems has demonstrated the importance of mi...
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Genetic and biochemical studies have previously implicated exonuclease 1 (Exo1) in yeast and mammalian mismatch repair, with results suggesting that function of the protein in the reaction depends on both its hydrolytic activity and its ability to interact with other components of the repair system. However, recent analysis of an Exo1-E109K knockin mouse has concluded that Exo1 function in mamm...
متن کاملMammalian mismatch repair: error-free or error-prone?
A considerable surge of interest in the mismatch repair (MMR) system has been brought about by the discovery of a link between Lynch syndrome, an inherited predisposition to cancer of the colon and other organs, and malfunction of this key DNA metabolic pathway. This review focuses on recent advances in our understanding of the molecular mechanisms of canonical MMR, which improves replication f...
متن کاملMeasurement of DNA mismatch repair activity in live cells.
Loss of DNA mismatch repair (MMR) function leads to the development and progression of certain cancers. Currently, assays for DNA MMR activity involve the use of cell extracts and are technically challenging and costly. Here, we report a rapid, less labor-intensive method that can quantitatively measure MMR activity in live cells. A G-G or T-G mismatch was introduced into the ATG start codon of...
متن کاملMismatch repair in heteroduplex DNA.
DNA with base pair mismatches was prepared by annealing mixtures of genetically marked DNA from bacteriophage lambda. This heteroduplex DNA was used to transfect bacteria under conditions minimizing recombination. Genetic analysis of the progeny phages indicates that: (i) Mismatch repair occurs, usually giving rise to a DNA molecule with one chain with the genotype arising from repair and one p...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2003
ISSN: 1362-4962
DOI: 10.1093/nar/gkg167